Patterning different cells based on microfluidics and self-assembled monolayers / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1234-1239, 2009.
Article
in Zh
| WPRIM
| ID: wpr-296933
Responsible library:
WPRO
ABSTRACT
The ability to pattern multiple cells through precise surface engineering of cell culture substrates has promoted the development of cellular bioassays, such as differentiation, interaction and molecular signaling pathways. There are several well developed ways to pattern cells. This report describes a method for patterning multiple types of cells based on microfluidics and self-assembled monolayers. We developed two types of micro-dam structures by soft-lithography to locate cells precisely and modified the substrate by a kind of self-assembled monolayer with property of electrochemical desorption to confine cells in specific areas. Finally we could pattern an array of two different types of cells closely and precisely. Cells were confined in specific areas but still shared the same microenvironment, so they could interact through soluble molecules. The substrate was transparent and open, so we could easily apply several instruments for research. With these merits, this cell chip is appropriate for investigating the interaction between different types of cells.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Physiology
/
Substrate Specificity
/
Umbilical Veins
/
Cell Adhesion
/
Cells
/
Cell Biology
/
Tissue Engineering
/
Cell Line, Tumor
/
Endothelial Cells
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article