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Expression of β-integrin family members in children with T-cell acute lymphoblastic leukemia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 620-626, 2017.
Article in Chinese | WPRIM | ID: wpr-297237
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of β-integrin family members in children with T-cell acute lymphoblastic leukemia (T-ALL) and their significance.</p><p><b>METHODS</b>Quantitative real-time PCR analyses were performed to assess the expression levels of β-integrin family members in bone marrow samples from 22 children with newly-diagnosed T-ALL and 21 controls (16 children with non-malignant hematologic disease and 5 healthy donors with bone marrow transplantation). Jurkat cells were treated with integrin inhibitor arginine-glycine-aspartate (Arg-Gly-Asp, RGD) peptide. The cell viability and apoptosis rate were determined by CCK8 assay and flow cytometry respectively.</p><p><b>RESULTS</b>The mRNA levels of integrins β, β, and βwere significantly lower in children with T-ALL than in controls (P<0.05). In T-ALL patients, high integrin βexpression was associated with lower white blood cell counts (<100×10/L), minimal residual disease (MRD) positivity, and day 33 bone marrow negative remission (P<0.05). In T-ALL patients, higher integrin βexpression was associated with relapse of T-ALL (P<0.05). Based on survival curve analysis, higher integrin βexpression was related to lower event-free survival and overall survival rates. RGD peptide treatment inhibited the proliferation of Jurkat cells and increased their apoptosis rate (P<0.05).</p><p><b>CONCLUSIONS</b>β-Integrin may play a role in the occurrence and development of T-ALL by affecting cell proliferation and apoptosis. The expression of integrin β5 is closely related to the risk of relapse of T-ALL. The expression of integrin β3 is closely related the treatment response and prognosis of T-ALL.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / RNA, Messenger / Mortality / Jurkat Cells / Integrin beta Chains / Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Genetics / Metabolism Type of study: Prognostic study Limits: Child / Child, preschool / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Contemporary Pediatrics Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / RNA, Messenger / Mortality / Jurkat Cells / Integrin beta Chains / Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Genetics / Metabolism Type of study: Prognostic study Limits: Child / Child, preschool / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Contemporary Pediatrics Year: 2017 Type: Article