Inhibitory effects of transfected Bcl-XL antisense oligodeoxynucleotide on proliferation of esophageal cancer cells and growth of human esophageal carcinoma in nude mice / 中华病理学杂志
Chinese Journal of Pathology
; (12): 402-406, 2005.
Article
in Zh
| WPRIM
| ID: wpr-297336
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biologic effects of Bcl-XL antisense oligodeoxynucleotide (ASODN) transfected into cultured esophageal carcinoma cells and human esophageal carcinoma xenograft in nude mice.</p><p><b>METHODS</b>Cationic liposome-mediated ASODN was used to transfect esophageal carcinoma cells. RT-PCR, Western blot, MTT assay, flow cytometry and in-situ apoptosis cells detection (TUNEL detection) were used to systematically study the biological effects of the transfected cells in vitro and in vivo.</p><p><b>RESULTS</b>MTT assay showed that the proliferation of esophageal carcinoma cells in the ASODN group decreased significantly as compared with control (P < 0.05), along with a 57.3% inhibitory rate of Bcl-XL mRNA, a significant decrease of Bcl-XL protein and the apoptosis rates of (31.1 +/- 5.8)% and 35.0% by flow cytometry and TUNEL assay, respectively (P < 0.01, as compared with controls). The growth of human esophageal carcinoma in nude mice was also significantly inhibited in the ASODN group (P < 0.05), along with a significant decrease of Bcl-XL mRNA and protein expression, and also an enhanced apoptosis of the tumor cells in nude mice.</p><p><b>CONCLUSIONS</b>Bcl-XL ASODN can effectively inhibit the proliferation of esophageal carcinoma cells in vitro and the growth of the tumor in vivo. The suppression of Bcl-XL expression by ASODN may offer both a therapeutic approach and an important theoretic foundation for gene therapy against esophageal carcinoma.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
RNA, Messenger
/
Esophageal Neoplasms
/
Transfection
/
Apoptosis
/
Oligodeoxyribonucleotides, Antisense
/
Cell Line, Tumor
/
Cell Proliferation
/
Bcl-X Protein
Limits:
Animals
/
Female
/
Humans
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2005
Type:
Article