Study on analgesia of oxymatrine and its relation to calcium channels / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 461-465, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-297406
ABSTRACT
<p><b>OBJECTIVE</b>To study whether the analgesis of oxymatrine (OMT) affects N-type voltage-gated calcium channels (VGCCs).</p><p><b>METHODS</b>Totally 45 mice were randomly divided into the sham-operation group, the model group [established by partial sciatic nerve ligation (PSNL)] , and the OMT treatment group according to random digit table, 15 in each group. The dorsal root ganglions (DRG) were separated in PSNL pain model mice. Intracellular calcium concentration ([Ca2+]i) was determined with Fluo-3 AM immunofluorescent probe in cultured DRG neurons. Different protein expression levels of N-type (Cav2. 2) and L-type ( Cav1. 3) among VGCCs from brain and DRG tissues were detected with Western blot.</p><p><b>RESULTS</b>Compared with the sham-operation group, [Ca2+]i, increased in cultured DRG neurons (P <0. 05) , protein expression levels of Cav2. 2 in the brain tissue increased (P <0. 05), protein expression levels of Cav2. 2 in DRG tissues decreased in the model group (P <0. 01). Compared with the model group, [Ca2+]i, decreased in cultured DRG neurons (P < 0. 05), protein expression levels of Cav2. 2 in the brain tissue decreased (P <0. 01), protein expression levels of Cav2. 2 in DRG tissues increased in the OMT treatment group (P <0. 01). There was no statistical difference in Cav1. 3 expressions in cultured DRG neurons and the brain (P >0. 05).</p><p><b>CONCLUSION</b>Analgesic effect of OMT might be related to Cav2. 2 channel mediated calcium ion flux.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pain
/
Pharmacology
/
Physiology
/
Quinolizines
/
Xanthenes
/
Calcium
/
Calcium Channels, N-Type
/
Alkaloids
/
Ganglia, Spinal
/
Analgesia
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2015
Type:
Article
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