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Effect of total saponins from rhizoma dioscoreae nipponicae on the expression of SDF1 and HCIKB kinase in rIK-1beta induced fibroblast-like synoviocytes / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine ; (12): 234-238, 2015.
Article in Chinese | WPRIM | ID: wpr-297421
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of total saponins from Rhizoma Dioscoreae nipponicae (RDN) on the expression of stroma cell derived factor 1 (SDF1) and IKB kinase (IKK) in rIL-1beta induced fibroblast-like synoviocytes (FLS).</p><p><b>METHODS</b>FLS were primarily cultured and the 3rd generation log phase growth FLS were divided into the normal control group, the model group, and the medication group. 10 microg/L rIL-1beta was used to induce the proliferation of FLS in the model group.10 microg/L rIL-1beta and 100 microg/L RDN were administered to co-incubate FLS in the medication group. No treatment was given to FLS in the normal control group. Expression levels of SDF1 and IkapaB kinase proteins (p-IKK) were detected using Western blot.</p><p><b>RESULTS</b>Expression levels of SDF1 and p-IKK increased significantly higher in the model group than in the normal control group (P<0.01). Compared with the model group, expression levels of SDF-1 and p-IKK significantly decreased in the medication group (P <0.01).</p><p><b>CONCLUSIONS</b>Total saponins from RDN could inhibit the activation of both SDF1 and p-IKK. It might further regulate the expression of IKB kinase by regulating the expression of SDF1.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Saponins / Synovial Membrane / Drugs, Chinese Herbal / Cells, Cultured / Dioscorea / Therapeutic Uses / Epithelial Cells / Interleukin-1beta / Chemokine CXCL12 Type of study: Prognostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Saponins / Synovial Membrane / Drugs, Chinese Herbal / Cells, Cultured / Dioscorea / Therapeutic Uses / Epithelial Cells / Interleukin-1beta / Chemokine CXCL12 Type of study: Prognostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Integrated Traditional and Western Medicine Year: 2015 Type: Article