Upregulation of P2X3 receptors in dorsal root ganglion of TRPV1 knockout female mice / 生理学报
Acta Physiologica Sinica
;
(6): 431-437, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-297474
ABSTRACT
The study was aimed to investigate the changes in mechanical pain threshold in the condition of chronic inflammatory pain after transient receptor potential vanilloid 1 (TRPV1) gene was knockout. Hind-paw intraplantar injection of complete freund's adjuvant (CFA, 20 μL) produced peripheral inflammation in wild-type and TRPV1 knockout female mice. The mechanical pain thresholds were measured during the 8 days after injection and pre-injection by using Von-Frey hair. Nine days after injection, mice were killed and the differences of expression of c-Fos and P2X3 receptor in the dorsal root ganglia (DRG) and spinal cord dorsal horn were examined by Western blotting between the two groups. Compared with that in wild-type mice, the mechanical pain threshold was increased significantly in TRPV1 knockout mice (P < 0.05); 3 days after CFA injection, the baseline mechanical pain threshold in the TRPV1 knockout mice group was significantly higher than that in the wild-type mice group (P < 0.05); The result of Western blotting showed that the expression of c-Fos protein both in DRG and spinal cord dorsal horn of TRPV1 knockout mice group was decreased significantly compared with that in wild-type mice group (P < 0.01, P < 0.05), while the expression of P2X3 receptor in DRG of TRPV1 knockout mice group was increased significantly compared with that in wild-type mice group (P < 0.05). Our findings indicate that TRPV1 may influence the peripheral mechanical pain threshold by mediating the expression of c-Fos protein both in DRG and spinal cord dorsal horn and changing the expression of P2X3 receptor in DRG.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pain
/
Spinal Cord
/
Up-Regulation
/
Proto-Oncogene Proteins c-fos
/
Pain Threshold
/
Mice, Knockout
/
TRPV Cation Channels
/
Receptors, Purinergic P2X3
/
Ganglia, Spinal
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2014
Type:
Article
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