Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells / 生理学报
Acta Physiologica Sinica
;
(6): 600-606, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-297532
ABSTRACT
The saturated free fatty acid (FFA), palmitate, could induce apoptosis in various cell types, but little is known about its effects on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). Here, we investigated whether palmitate induced apoptosis and endoplasmic reticulum (ER) stress in hUC-MSCs. hUC-MSCs were stained by labeled antibodies and identified by flow cytometry. After administration with palmitate, apoptotic cell was assessed by flow cytometry using the Annexin V-FITC/7-AAD apoptosis detection kit. Relative spliced XBP1 levels were analyzed using semi-quantitative RT-PCR. The mRNA of BiP, GRP94, ATF4 and CHOP were analyzed by real-time PCR. Relative BiP and CHOP protein were analyzed using Western blot analysis. The results showed that hUC-MSCs were homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs and activated XBP1 splicing, BiP, GRP94, ATF4 and CHOP transcription. These findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Palmitates
/
Pharmacology
/
Transcription Factors
/
Umbilical Cord
/
Membrane Glycoproteins
/
Apoptosis
/
Cell Biology
/
DNA-Binding Proteins
/
Activating Transcription Factor 4
/
Transcription Factor CHOP
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2013
Type:
Article
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