Involvement of store-operated calcium channels and receptor-operated calcium channels in Ca(2+)-sensing receptor-evoked extracellular Ca(2+) influx and NO generation in human umbilical vein endothelial cells / 生理学报
Acta Physiologica Sinica
;
(6): 553-561, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-297537
ABSTRACT
This paper aims to investigate the effect of store-operated calcium channels (SOC) and receptor-operated calcium channels (ROC) on Ca(2+)-sensing receptor (CaR)-induced extracellular Ca(2+) influx and nitric oxide (NO) generation in human umbilical vein endothelial cells (HUVEC). SOC blocker, non-selective cation channel blocker, ROC agonist and ROC blocker were used separately and combined. Intracellular Ca(2+) concentration ([Ca(2+)]i) was measured by Fura-2/AM loading. The activity of endothelial nitric oxide synthase (eNOS) and the production of NO were determined by the DAF-FM diacetate (DAF-FM DA). The results showed that increases of [Ca(2+)]i, eNOS activity and NO generation induced by CaR agonist Spermine were all reduced after single blocking the SOC or ROC, respectively (P < 0.05). ROC agonist can partially abolish the ROC blocker's effect (P < 0.05). The above mentioned effects evoked by CaR agonist Spermine were further reduced when blocking both SOC and ROC than single blocking SOC or ROC in HUVEC (P < 0.05). In conclusion, these results suggest that the SOC and ROC participate in the processes of CaR-evoked extracellular Ca(2+) influx and NO generation by a synergistic manner in HUVEC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Calcium Channel Blockers
/
Calcium Channels
/
Calcium
/
Calcium Signaling
/
Receptors, Calcium-Sensing
/
Nitric Oxide Synthase Type III
/
Human Umbilical Vein Endothelial Cells
/
Fluoresceins
Limits:
Humans
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2013
Type:
Article
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