Effects of sodium arsenite on catalase in human keratinocytes / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 605-607, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-297634
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT).</p><p><b>METHODS</b>The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT.</p><p><b>RESULTS</b>If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05).</p><p><b>CONCLUSION</b>CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Catalase
/
Keratinocytes
/
Cell Line
/
Blotting, Western
/
Sodium Compounds
/
Arsenites
/
Reverse Transcriptase Polymerase Chain Reaction
/
Dose-Response Relationship, Drug
/
Toxicity
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2006
Type:
Article
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