Chinese herbal medicine PC-SPES II induces the apoptosis of androgen-independent prostate carcinoma cell line PC-3 / 中华男科学杂志
National Journal of Andrology
;
(12): 563-567, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-297683
ABSTRACT
<p><b>OBJECTIVE</b>To study the action mechanism of a Chinese herbal mixture PC-SPES II inducing the apoptosis of androgen independent prostate adenocarcinoma cell line (PC-3).</p><p><b>METHODS</b>The growth of PC-3 was shown by MTT. Immunofluorescence staining of acridine orange (AO) and flow cytometry were used to detect the apoptosis. The expressions of the apoptosis-related proteins were analyzed with their monoclonal or polyclonal antibodies after Western blotting.</p><p><b>RESULTS</b>PC-SPES II not only inhibited the growth of PC-3 cells but also induced their death. The apoptosis of PC-3 cells treated with PC-SPES II was detected by immunofluorescence staining of AO and flow cytometry, which showed the apoptotic cells to be (29.8 +/- 5.6)%, but the untreated control cells (0.06 +/- 0.014)%, (P < 0.01). The expression of Bcl-2 and Bcl-xL, two antiapoptosis proteins, was decreased while Bax, a pro apoptosis protein, was elevated in the cells treated with PC-SPES II as compared with the untreated control (P < 0.01). Accordingly, the expression of the activated fragments of caspase-3 was also increased (P < 0.01).</p><p><b>CONCLUSION</b>The Chinese herbal mixture PCSPES II can induce the apoptosis of PC-3. Its mechanism may lie in the up-regulation of Bax expression and down-regulation of Bcl-2 and Bcl-xL expressions, which induce the activated fragments of caspase-3 by mitochondria.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Prostatic Neoplasms
/
Drugs, Chinese Herbal
/
Apoptosis
/
Cell Line, Tumor
/
Therapeutic Uses
/
Drug Therapy
/
Phytotherapy
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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