Voltage-dependent anion channels (VDAC) on human sperm membrane / 中华男科学杂志
National Journal of Andrology
;
(12): 498-501, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-297697
ABSTRACT
<p><b>OBJECTIVE</b>To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.</p><p><b>METHODS</b>VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.</p><p><b>RESULTS</b>The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.</p><p><b>CONCLUSION</b>VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Spermatozoa
/
Testis
/
Recombinant Proteins
/
Signal Transduction
/
Gene Expression
/
Blotting, Western
/
Polymerase Chain Reaction
/
Voltage-Dependent Anion Channels
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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