Hypoxia promotes apoptosis of germ cells in rat testes / 中华男科学杂志
National Journal of Andrology
;
(12): 487-491, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-297699
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of hypobaric hypoxia on the apoptosis of germ cells in male rats.</p><p><b>METHODS</b>Adult male Wistar rats were randomly divided into four groups a control group raised at sea level; a 5 d, a 15 d and a 30 d hypoxic group raised in a hypobaric chamber simulating 5000 m altitude for 5 days, 15 days and 30 days respectively. Flow cytometry and TUNEL were used to evaluate the apoptosis of germ cells in the testis. Bax and Bcl-2 in the testis were measured by Western blot.</p><p><b>RESULTS</b>Seminiferous tubules with apoptotic germ cells were significantly more in the hypoxic groups than in the control (P < 0.01). Most apoptotic germ cells were spermatogonia and spermatocytes. Compared with the control group, apoptotic germ cells detected by PI flow cytometry were significantly increased in the hypoxic 15 d and 30 d groups (P < 0.05); Bax was significantly higher (P < 0.05), and so was the ratio of Bax to Bcl-2 in the hypoxic 30 d group (P < 0.01).</p><p><b>CONCLUSION</b>Hypoxia promotes apoptosis of testicular germ cells in male rats. Chronic hypoxia increases Bax expression in the rat testis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Spermatozoa
/
Testis
/
Random Allocation
/
Rats, Wistar
/
Apoptosis
/
In Situ Nick-End Labeling
/
Cell Biology
/
Bcl-2-Associated X Protein
/
Hypoxia
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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