Effect of urokinase-type plasminogen activator on the mitochondrial membrane potential of mouse capacitated-spermatozoa in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 391-395, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-297719
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of uPA improving sperm capacitation by investigating the effect of uPA on the mitochondrial function of mouse capacitated-sperm in vitro.</p><p><b>METHODS</b>Mitochondrial function of mouse capacitated-spermatozoa was evaluated through the assessment of mitochondrial membrane potential using JC-1 performed by flow cytometer and fluorescent microscope respectively. The experiment and the control groups were designed according to the presence or absence of uPA, each divided into 5 subgroups based on the different time of uPA treatment (or BWW in the control groups) at 0, 5, 15, 30 and 60 min respectively.</p><p><b>RESULTS</b>(1) Compared with that at 0 min, the mean fluorescence intensity of JC-1 within the spermatozoal body and the percentage of orange-red colored spermatozoa in the experiment group were increased significantly at 5 and 15 min respectively after uPA incubation (P < 0.05). (2) The mean fluorescence intensity of JC-1 within the spermatozoal body at 15, 30 and 60 min and the percentage of orange-red colored spermatozoa at 5 and 15 min in the group were significantly higher than those in the control group (P < 0.05).</p><p><b>CONCLUSION</b>uPA could increase the mitochondrial membrane potential of mouse capacitated-spermatozoa in vitro, and maintain it at a high level for a certain period of time. By enhancing sperm mitochondrial function, uPA may provide sufficient energy for capacitated-spermatozoa to increase their motility and change their motor pattern, which might be one of the therapeutic mechanisms of uPA on male infertility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Sperm Capacitation
/
Sperm Motility
/
Spermatozoa
/
Urokinase-Type Plasminogen Activator
/
Membrane Potential, Mitochondrial
/
Flow Cytometry
/
Fluorescent Dyes
/
Mice, Inbred Strains
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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