Detection of AMACR (P504S), P63 and 34betaE12 cocktail in the early diagnosis of prostate cancer / 中华男科学杂志
National Journal of Andrology
;
(12): 222-225, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-297750
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the value of detection of AMACR (P504S), P63 and 34betaE12 cocktail in the early diagnosis of prostate cancer (PCa).</p><p><b>METHODS</b>The expressions of AMACR, P63 and 34betaE12 were examined in the biopsy specimens of 42 cases of prostate cancer, 12 cases of high-grade prostatic intraepithelial neoplasia (HGPIN) and 30 cases of benign prostatic hyperplasia (BPH) using the Maxvision single-step immunohistochemical method with triple-antibody cocktail (AMACR/P63/34betaE12) staining and double-color chromogens in single paraffin sections .</p><p><b>RESULTS</b>The expressions of AMACR, P63 and 34betaE12 were significantly different between PCa and BPH (P < 0.01). The staining of PCa was positive for AMACR and negative for P63 and 34betaE12, and the positivity rate of AMACR was 100%. BPH was strongly expressed for P63 and 34betaE12, but negatively for AMACR. The expression of AMACR was significantly different between HGPIN and BPH (P < 0.01), but not between HGPIN and PCa (P > 0.05), and the positivity rate of AMACR in HGPIN was 91.67%. However, the expressions of P63 and 34betaE12 were significantly different between HGPIN and PCa (P < 0.01), but not between HGPIN and BPH (P > 0.05), and the positivity rate of AMACR in HGPIN was 100%. The level of AMACR expression was not correlated with PCa Gleason score (P > 0.05).</p><p><b>CONCLUSION</b>AMACR is a sensitive and specific marker for PCa. P63 and 34betaE12 cocktail staining can increase the sensitivity and specificity of the basal cell detection. The immunohistochemical analysis with triple-antibody cocktail (AMACR/P63/34betaE12) staining and double-color chromogens can improve diagnostic accuracy and has an important applied value for the early diagnosis of prostate cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostatic Hyperplasia
/
Prostatic Neoplasms
/
Immunohistochemistry
/
Carcinoma, Basal Cell
/
Racemases and Epimerases
/
Early Diagnosis
/
Diagnosis
/
Keratins
/
Membrane Proteins
/
Metabolism
Type of study:
Diagnostic study
/
Screening study
Limits:
Aged
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Type:
Article
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