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ShRNA against NSP4 gene inhibits the proliferation of bovine rotavirus in vitro / 病毒学报
Chinese Journal of Virology ; (6): 244-248, 2010.
Article in Chinese | WPRIM | ID: wpr-297876
ABSTRACT
Based on the NSP4 sequence of bovine rotavirus (BRV), the shRNA was designed and synthesized, and a shRNA recombinant lenti-virus vector RNAi-H1-89 was constructed. The recombinant RNAi-H1-89 Lenti-virus was packaged by transfecting the 293T cell with the recombinant vector RNAi-H1-89 and two helper plasmids using lipofectamine, and then used to infect MA104 cells. The MA104 cells were further infected with BRV strain G6 24h post-infection, with the LacZ shRNA recombinant lenti-virus as control. Thirty-six hours later, the CPE of the infected cells was observed under microscope, shRNA of NSP4 gene inhibited CPE in MA104 cell; the shRNA against NSP4 gene also inhibited NSP4 gene expression by RT-PCR, The virus titer in the cell culture supernatant was significant lower compared with the control group. The above results showed that RNAi-H1-89 against NSP4 gene could specifically silence NSP4 gene expression, and inhibit the proliferation of BRV.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Plasmids / Toxins, Biological / Virus Replication / DNA, Recombinant / Molecular Sequence Data / Base Sequence / Glycoproteins / Cell Line / Viral Nonstructural Proteins Limits: Animals Language: Chinese Journal: Chinese Journal of Virology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Plasmids / Toxins, Biological / Virus Replication / DNA, Recombinant / Molecular Sequence Data / Base Sequence / Glycoproteins / Cell Line / Viral Nonstructural Proteins Limits: Animals Language: Chinese Journal: Chinese Journal of Virology Year: 2010 Type: Article