Genome sequencing and analysis of the bovine viral diarrhea virus-2 strain JZ05-1 isolated in China / 病毒学报
Chinese Journal of Virology
;
(6): 238-243, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-297877
ABSTRACT
Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus, which is a widespread problem for beef and dairy herds, and has given rise to a significant loss in the livestock industry all over the world. The BVDV strain JZ05-1 isolated from cattle in Jilin, China generated cytopathic effect (CPE) in MDBK cells. Eight overlapped gene fragments were amplified by RT-PCR and sequenced, the complete genom sequence of BVDV strain JZ05-1 was assembled. According to the results, the JZ05-1 genome was composed of 12285 nucleotides in length (GenBank accession No. GQ888686), which could be divided into three regions a 387 nt 5'-untranslated region (UTR), a 11694 nt single large open reading frame encoding a polyprotein, and a 204 nt 3'-UTR. The 5'-UTR and genome sequences were analyzed by sequence alignment and construction of phylogenetic trees. The strain JZ05-1 was classified as BVDV type 2a. The BVDV-2 strain JZ05-1 genome showed high similarity to the p11Q isolated in Canada and the XJ-04 isolated in China, with 90% and 91% identity in nucleotide sequence, respectively. Compared with the similarity within the BVDV-2 genotype (96%), the JZ05-1 had low sequence similarity to other BVDV-2 strains.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Molecular Sequence Data
/
China
/
Classification
/
Genome, Viral
/
Sequence Analysis, DNA
/
5' Untranslated Regions
/
Reverse Transcriptase Polymerase Chain Reaction
/
Diarrhea Virus 2, Bovine Viral
/
Genetics
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2010
Type:
Article
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