Simultaneous detection of measles and rubella virus by multiplex real-time RT-PCR with an internal control / 病毒学报
Chinese Journal of Virology
;
(6): 109-114, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-297898
ABSTRACT
Measles and rubella virus cause fever rash diseases that are uneasy to differentiate clinically from each other. Specific primers and fluorescence-labeled probes were designed, and a multiplex Real-time RT-PCR with an internal control was developed to simultaneously identify the measles and rubella virus. The multiplex Real-time RT-PCR assay was specific and no false positive or false negative results were found. The sensitivity of the assay was 0.1TCID50/mL and 1TCID50/mL for measles and rubella virus respectively. Analysis with 0.1-10(3)TCID50/mL measles or rubella virus samples demonstrated high validity and reproducibility with the coefficient of variability(CV) of below 0.9% for both measles and rubella virus. Using ribonuclease P (RNase P) as internal false negative control, the developed multiplex Real-time RT-PCR assay is suitable for rapid clinical diagnosis of measles and rubella virus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rubella
/
Rubella virus
/
Virology
/
RNA, Viral
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Reverse Transcriptase Polymerase Chain Reaction
/
Ribonuclease P
/
Diagnosis
/
Fluorescent Dyes
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2010
Type:
Article
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