Quasispecies investigation on swine hepatitis E viruses / 病毒学报
Chinese Journal of Virology
;
(6): 40-44, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-297920
ABSTRACT
The objective of current study was to investigate the quasispecies of hepatitis E virus in swine. The partial ORF2 region of HEV envelope gene from four swine HEV strains was amplified by RT-nested polymerase chain reaction (RT-nPCR). After cloning and transformation of PCR products, 20 positive clones of each HEV isolate were subject to sequencing and DNA analysis. The homology among the different clones of each isolates was 96.8%-99.7%, 98.8%-99.7%, 98.8%-99.7% and 100%, respectively, while there was 96.8%-100% sequence identity at the nucleotide level compared with HEV strains isolated in Shanghai (SAAS-JDY5). This study confirmed that there existed quasispecies of HEV in swine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Swine
/
Virology
/
Disease Reservoirs
/
Base Sequence
/
Chemistry
/
China
/
Open Reading Frames
/
Sequence Alignment
/
Amino Acid Sequence
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2010
Type:
Article
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