Quantitative determination of bosentan by proton nuclear magnetic resonance with internal standard method / 药学学报
Acta Pharmaceutica Sinica
;
(12): 249-251, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-297985
ABSTRACT
The study aims to establish a quantitative nuclear magnetic resonance (QNMR) method for the determination of the absolute content of bosentan. Proton nuclear magnetic resonance spectroscopy [1H NMR] spectra were obtained in CDCl3 with the internal standard dimethyl terephthalate and zg30 pulse sequence by using a Bruker AVANCE II 400 spectrometer. The content of bosentan is determined with QNMR in comparison with the result obtained by mass balance method. The result is 96.25% by QNMR and 96.54% by mass balance method. A rapid and accurate QNMR method has been established for the quantitative determination of the absolute content of bosentan. The study provides a new way for the quality control and calibration of a new reference standard material, it could be the complementary with the mass balance method for the assay of standard reference.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protons
/
Quality Control
/
Sulfonamides
/
Calibration
/
Magnetic Resonance Spectroscopy
/
Molecular Structure
/
Chemistry
/
Methods
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2014
Type:
Article
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