Sulfated modification and anticoagulant activity in vitro of sulfated glucan isolated from the aqueous extract of Hedysarum polybotrys / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1665-1670, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-298028
ABSTRACT
SHG was sulfated by chlorosulfonic acid-pyridine method, and six samples which we got were prepared in different reaction conditions. There is a characteristic absorption peak near 260 nm in UV spectra and there are two characteristic absorption peaks near 1240 cm(-1) and 810 cm(-1) in the FT-IR. Degree of sulfation (DS) was calculated by elemental analysis and turbidimetry. Under the same conditions the absorption peaks become strong with the DS increase. The anticoagulant activity of SHG and sulfated modification samples was evaluated by the classic coagulant assays of prothrombin time (PT), activated partial thrombin time (APTT) live enzymes, and plasma thrombin time (TT). Results show that sulfated SHG has a good anticoagulant activity in vitro, and DS increased activity within a certain range.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Partial Thromboplastin Time
/
Pharmacology
/
Plants, Medicinal
/
Prothrombin Time
/
Spectrophotometry, Ultraviolet
/
Sulfonic Acids
/
Thrombin Time
/
Blood Coagulation Tests
/
Chemistry
/
Spectroscopy, Fourier Transform Infrared
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2013
Type:
Article
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