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Minimally modified LDL induced impairment of endothelium-dependent relaxation in mesenteric arteries of mice / 药学学报
Acta Pharmaceutica Sinica ; (12): 1657-1664, 2013.
Article in Chinese | WPRIM | ID: wpr-298029
ABSTRACT
This study is to investigate the impairment and possible mechanism of endothelium-dependent relaxation of mice mesenteric arteries induced by mmLDL. Wire myography was employed to examine endothelial function of mesenteric arteries. Ultramicrostructure of mesenteric vascular beds were detected by transmission electron microscope. The results showed that endothelium cell edema and peeling, vascular elastic membrane fracture traces in mmLDL group. Endothelium-dependent relaxation was decreased in a time-dependent and dose-dependent manner by using mmLDL, compared with normal arteries. In endothelium-derived hyperpolarizing factor (EDHF)-mediated relaxation, the Rmax and pIC50 were decreased from (63 +/- 5) % and 6.42 +/- 0.09 of normal saline control to (31 +/- 3) % and 5.67 +/- 0.07 in mmLDL group (P < 0.001, P < 0.001), respectively. In nitric oxide (NO)-mediated relaxation, the Rmax and pIC50 were decreased from (45 +/- 4) % and 5.93 +/- 0.08 in normal saline control to (32 +/- 4) % and 5.43 +/- 0.11 in mmLDL group (P < 0.05, P < 0.01), respectively. There is no significant alteration of prostacyclin I2 (PGI2) pathway between these two groups. In conclusion, mmLDL induced the impairment of the ultramicrostructure of mesenteric vascular endothelium cell as well as the endothelium-dependent relaxation. The latter includes the dysfunction of NO- and EDHF pathway mediated endothelium-dependent relaxation.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Vasodilation / Biological Factors / Epoprostenol / Cell Biology / Endothelial Cells / Microscopy, Electron, Transmission / Dose-Response Relationship, Drug / Lipoproteins, LDL Limits: Animals Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Vasodilation / Biological Factors / Epoprostenol / Cell Biology / Endothelial Cells / Microscopy, Electron, Transmission / Dose-Response Relationship, Drug / Lipoproteins, LDL Limits: Animals Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2013 Type: Article