Antibody preparation and enzyme linked immunosorbent assay of Lonicera japonica phenylalnine ammonia lyase / 药学学报
Acta Pharmaceutica Sinica
; (12): 1498-1502, 2013.
Article
in Zh
| WPRIM
| ID: wpr-298052
Responsible library:
WPRO
ABSTRACT
The expression of phenylalnine ammonia lyase (LJPAL1) is closely related to the content of active compounds in Lonicera japonica. In this paper, a prokaryotic expression vector is constructed and LJPAL1 protein is expressed in E. coli. Three antigen sites were synthesized to peptide antigen and prepared polyclonal antibody of Anti-LJT-1, Anti-LJT-2 and Anti-LJT-3, separately. Antibody Anti-LJT-2 was screened using Western blotting. And indirect ELISA was built using Anti-LJT-2. The results of this study will be a base for honeysuckle chemical quality and evaluation kits.
Full text:
1
Index:
WPRIM
Main subject:
Phenylalanine Ammonia-Lyase
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Plants, Medicinal
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Plasmids
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Enzyme-Linked Immunosorbent Assay
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Chemistry
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Blotting, Western
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Protein Structure, Tertiary
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Protein Structure, Secondary
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Gene Expression Regulation, Plant
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Lonicera
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2013
Type:
Article