Construction of lentivirus producing PLC-gamma1 siRNA and its effect on apoptosis of human colorectal carcinomas cell lines / 南方医科大学学报
Journal of Southern Medical University
; (12): 255-258, 2007.
Article
in Zh
| WPRIM
| ID: wpr-298193
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct recombinant lentivirus that stably suppresses phospholipase C (PLC) gamma1 expression in human colorectal carcinoma LoVo cells to obtain LoVo cell lines deficient in PLC gamma1 for investigation of the role of PLC gamma1 gene.</p><p><b>METHODS</b>Recombinant lentivirus producing PLC gamma1 siRNA were constructed to infect LoVo cells, and the stably transduced cells were selected with blasticidin. The protein and mRNA expression of PLC gamma1 was examined by Western blotting and RT-PCR, and the effect of the lentivirus on cell apoptosis was analyzed by flow cytometry.</p><p><b>RESULTS AND CONCLUSION</b>PLC gamma1 siRNA significantly suppressed PLC gamma1 expression in LoVo cells, suggesting high efficiency of gene silencing induced by the siRNA produced by the recombinant lentivirus. Concomitantly, cell apoptosis induced by 5-FU was significantly increased.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
DNA, Recombinant
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RNA, Messenger
/
Transfection
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Colorectal Neoplasms
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Blotting, Western
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Apoptosis
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Lentivirus
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Reverse Transcriptase Polymerase Chain Reaction
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article