Protective effect of donator liver ischemic preconditioning on posttransplant liver graft function in Chinese miniature pigs / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the protective effect of the ischemia preconditioning of the donor liver on posttransplant liver graft function in Chinese miniature pigs.</p><p><b>METHODS</b>Twenty-five partially inbred Chinese miniature pigs were randomized into three groups, namely the normal control group, the ischemia-reperfusion group and ischemic preconditioning group. Biopsies of the liver graft were performed to analyze HSP70 expression by means of immunoblotting, and the changes of serum AST/ALT levels were assayed using an automated biochemical analyzer. Histopathological assessment was carried out to identify the hepatocyte injury using optical and transmission electron microscopy.</p><p><b>RESULTS</b>Ischemia preconditioning resulted in a notable increase in HSP70 expression and milder injury of the hepatocyte microstructure, whereas ischemia-reperfusion caused a significant increase of serum transaminases level (P<0.01) with declined HSP70 expression and obvious microstructural changes of the liver tissue.</p><p><b>CONCLUSION</b>Ischemic preconditioning can produce obvious protective effects on the donor liver, and positively regulates the expression of shock protein.</p>