Expression of bcl-2 gene in EBV-transformed human gastric epithelial cell line GES-1 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 195-197, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-298207
ABSTRACT
<p><b>OBJECTIVE</b>To detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.</p><p><b>METHODS</b>Akata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418. Immunocytochemical staining was performed to detect the expressions of EBNA1 and Bcl-2 protein.</p><p><b>RESULTS</b>Bcl-2 protein expression was detected in EBV-infected cells but not in the control cells.</p><p><b>CONCLUSION</b>EBV infection can increase Bcl-2 expression in gastric epithelial cells, and such cell transformation effect of EBV is related to the overexpression of bcl-2 gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach
/
Virology
/
Cell Line, Transformed
/
Immunohistochemistry
/
Cell Transformation, Viral
/
Herpesvirus 4, Human
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Biology
/
Epithelial Cells
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS