Modification of vascular endothelial cell treatment for patch clamp study / 南方医科大学学报
Journal of Southern Medical University
;
(12): 175-180, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-298213
ABSTRACT
A modified protocol for quick and economic treatment of cultured human umbilical vein endothelial cells has been established, which result in high viability of the cells to allow better performance in patch-clamp studies. The electrophysiological properties of Ca (2+)-activated K(+) (KCa) channel of the cultured cells were investigated with a cell-attached configuration. With this modified treatment method, the cultured cells appear fusiform in shape under microscope, and KCa channel currents could be detected readily, suggesting their eligibility for patch-clamp studies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Cells, Cultured
/
Reproducibility of Results
/
Patch-Clamp Techniques
/
Cell Biology
/
Potassium Channels, Calcium-Activated
/
Endothelial Cells
/
Membrane Potentials
/
Methods
Type of study:
Practice guideline
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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