Analysis of monosaccharides in Radix Rehmanniae by GC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 419-422, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-298388
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and purify the polysaccharides from Radix Rehmanniae and analysis the monosaccharides composition.</p><p><b>METHOD</b>The polysaccharides were extracted with hot water and precipitated by alcohol. Proteins in the precipitates were removed by TCA method. The products were further purified with column chromatography on Superdex 200 and Sephadex G100. The SRP I and SRP II were identified as homogeneous polysaccharide by HPLC, respectively, and then analyzed by GC after being hydrolysised.</p><p><b>RESULT</b>Two homogeneous polysaccharides (SRP I and SRP II) were obtained from Radix Rehmanniae.</p><p><b>CONCLUSION</b>SRP I contained rhamnose, arabinose, glucose and galactose in the percentage of 6.11%, 66.46%, 3.93% and 21.50%. SRP I was composed of rhamnose, fucose, mannose, galactose and fructose in the percentage of 21.82%, 24.47%, 10.48%, 29.94% and 13.29%.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arabinose
/
Polysaccharides
/
Rhamnose
/
Drugs, Chinese Herbal
/
Plant Extracts
/
Chemistry
/
Chromatography, Gas
/
Clinical Laboratory Techniques
/
Scrophulariaceae
/
Fructose
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2009
Type:
Article
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