Treatment of mouse liver metastasis by intraportal injection of Adv-p53 / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 818-821, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-298504
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of intraportal administration of Adv-p53 in the treatment of the liver metastasis in mice.</p><p><b>METHODS</b>2 x 10(5) of MCA-205 cells were injected into the mouse portal vein to establish a murine liver metastasis model. The spleen was transpositioned subcutaneously to enable the administration of Adv-p53 continually into the portal system. Different doses of Adv-p53 were injected intraportally, while HBSS and Adv-CMV were injected intraportaly in the control group. Tumors in the liver were examined on day 21 after Adv-p53 administration.</p><p><b>RESULTS</b>The liver weight in the Adv-p53 treated mice on day 0 group (1.20 +/- 0.34 g) was significantly less than that in the Adv-CMV group (2.59 +/- 0.48 g, P < 0.05). The number of metastatic nodules in the Adv-p53 treated mice on day 0 group (9.0 +/- 9.9) was significantly less than that in the Adv-CMV group (57.1 +/- 11.3, P < 0.05), indicating that intraportal administration of Adv-p53 inhibited the formation of liver metastasis. This anti-tumor effect was in a dose-dependent manner. After the liver metastasis was formed, Adv-p53 was administered intraportally. The liver weight in the Adv-p53 treated mice on day 5 group (1.22 +/- 0.09 g) was significantly less than that in the Adv-CMV group (3.98 +/- 1.01 g , P < 0.05). The number of metastatic nodules in the Adv-p53 treaed mice on day 5 group (5.5 +/- 3.5) was significantly less than that in the Adv-CMV group (113.2 +/- 5.8, P < 0.05). Repeatedly intraportal administration of Adv-p53 could enhance this anti-tumor effect.</p><p><b>CONCLUSION</b>Local administration of Adv-p53 into the portal system would be a useful strategy for the liver metastasis treatment.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Therapeutics
/
Recombinant Proteins
/
Genetic Therapy
/
Adenoviridae
/
Tumor Suppressor Protein p53
/
Cell Line, Tumor
/
Therapeutic Uses
/
Dose-Response Relationship, Drug
/
Fibrosarcoma
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article
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