Expression of tumor suppressor gene PRDM1 in lung cancers / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 434-436, 2007.
Article
in Zh
| WPRIM
| ID: wpr-298582
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the expression of tumor suppressor gene PRDM1 in lung cancers.</p><p><b>METHODS</b>Forty-five cases were enrolled in this study, including squamous cell carcinoma (20 cases), adenocarcinoma (15 cases), and small cell cancer (10 cases). PRDM1 protein was detected in paraffin-embedded tissue by immunohistochemistry. Tumor cells in lung cancers were further selected by laser microdissection for RT-PCR analysis. PRDM1 protein in frozen tissue was also detected by Western blot.</p><p><b>RESULTS</b>(1) PRDM1 protein was found in paraffin-embedded tissues in 90.0% (18/20) of squamous cell carcinoma, 13.3% (2/15) of adenocarcinoma, and 0 (0/10) small cell lung cancer. Squamous cell carcinoma predominantly expressed PRDM1 protein ( P < 0.01). (2) Gene product of PRDM1 DNA binding region was not found in microdissected tumor cells, but an abnormal PRDM1 protein about 70 KD was detected simultaneously in whole tumor tissue.</p><p><b>CONCLUSION</b>PRDM1 may be considered as a specific biomarker in pulmonary squamous cell carcinoma. The abnormal PRDM1 expression both at transcriptional and protein levels indicated that this tumor suppressor gene lost its function, which may become a new target in the strategy of treatment for lung cancers.</p>
Full text:
1
Index:
WPRIM
Main subject:
Repressor Proteins
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Transcription Factors
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Immunohistochemistry
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Carcinoma, Squamous Cell
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Adenocarcinoma
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Biomarkers, Tumor
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Gene Expression Regulation, Neoplastic
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Blotting, Western
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Carcinoma, Small Cell
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Reverse Transcriptase Polymerase Chain Reaction
Limits:
Adult
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Aged
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2007
Type:
Article