Experimental BCSC-1 gene therapy on nasopharyngeal carcinoma mediated by adenovirus / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 208-210, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-298711
ABSTRACT
<p><b>OBJECTIVE</b>To explore the therapeutic value of BCSC-1 in tumor gene therapy.</p><p><b>METHODS</b>Recombinant adenovirus Ad5-BCSC-1 was prepared. Cell proliferation was assayed using CellTiter 96 Aqueous one solution cell proliferation assay kit. Ad5-BCSC-1 was injected into tumor with Ad5-egfp or with PBS injection as controls. The injections were repeated one weak later. After another 2 weeks, the mice were sacrificed and the tumors were excised and weighed.</p><p><b>RESULTS</b>The growth of the CNE-2L2 cell infected with Ad5-BCSC-1 in vitro was remarkably slower than that of the controls, the wild type cell and the cell infected with Ad5-egfp. In contrast to the controls, the cells infected with Ad5-BCSC-1 aggregated and formed huge messes in the culture. The average weight of the CNE-2L2 tumors in mice was (2.28 +/- 0.73), (2.07 +/- 0.40), and (0.58 +/- 0.32) g for the tumors injected with PBS, Ad5-egfp, and Ad5-BCSC-1, respectively (Ad5-BCSC-1 vs. PBS or Ad5-egfp, P<0.05).</p><p><b>CONCLUSION</b>Intra-tumor injection of Ad5-BCSC-1 can suppress the growth of CNE-2L2 tumor in nude mice, suggesting that BCSC-1 gene therapy may be effective for tumors with low or no expression of BCSC-1 gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Therapeutics
/
Carcinoma
/
Genetic Therapy
/
Adenoviridae
/
Nasopharyngeal Neoplasms
/
Xenograft Model Antitumor Assays
/
Cell Line, Tumor
/
Genetic Vectors
/
Genetics
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2008
Type:
Article
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