Effect of genistein on MAPK signal pathway in the collagen-induced arthritis fibroblast-like synoviocytes / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1405-1408, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-298994
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of genistein (Gen) on MAPK signal pathway in the CIA rat fibroblast-like synoviocytes (FLS).</p><p><b>METHODS</b>The rat model of collagen-induced arthritis (CIA) was established. The cultured FLS of CIA rats were divided using randomized method. The effects of Gen (at the concentration of 50, 100, and 200 micromol/L, respectively) on the proliferation of FLS in CIA rats using methyl thiazolyl tetrazolium (MTT) assay. Effects of Gen (at the concentration of 50, 100, and 200 pmol/L, respectively) on the expressions of extracellular signal-regulated kinase (ERK) and phosphorylated extracellular signal-regulated kinase (p-ERK) in the FLS of CIA rats were detected.</p><p><b>RESULTS</b>Gen could inhibit the proliferation of FLS in CIA rats. The FLS proliferation in the high dose Gen group at 72 h was only 1.10+/-0.04, significantly lower than that in the model group (2.12+/-0.03, P<0.01). Besides, after Gen's action on FLS, the expression of p-ERK was down-regulated. It was only 0.34+/-0.02 in the high dose Gen group, significantly lower than that in the model group (2.68+/-0.14, P<0.01). There was no change in the expression of ERK (P>0.05).</p><p><b>CONCLUSIONS</b>Gen could inhibit the proliferation of FLS in CIA rats. Its mechanism of action was mainly correlated to down-regulating the tyrosine kinase of MAPK signal transduction pathway and inhibiting phosphorylation of ERK.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Arthritis, Experimental
/
Synovial Membrane
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Genistein
/
MAP Kinase Signaling System
/
Cell Biology
/
Extracellular Signal-Regulated MAP Kinases
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2011
Type:
Article
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