Interaction of E3 ligase HUWE1 and eukaryotic translation initiation factor eIF4E / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1543-1546, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-299100
ABSTRACT
To explore the regulation of eIF4E, we screened the protein interacting with eIF4E from human cDNA library by using yeast two-hybrid system. Several clones interacting with eIF4E were identified. One of them was homologous with HUWE1 (HECT, UBA and WWE domain containing 1, also named as ARF-BP1, HECTH9 or HUWE1). Cell co-immunoprecipitation showed that eIF4E could bind to HUWE1 in mammalian cells. We also found that HUWE1 bearing the HECT domain is necessary for its association with eIF4E.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Eukaryotic Initiation Factor-4E
/
Ubiquitin-Protein Ligases
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2014
Type:
Article
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