Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1446-1450, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-299113
ABSTRACT
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bufanolides
/
Bufonidae
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Tandem Mass Spectrometry
/
Amphibian Venoms
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2014
Type:
Article
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