Differential expression of osteopontin and bax, bcl-2 by fluoride / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 107-110, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-299188
ABSTRACT
<p><b>OBJECTIVE</b>To study the differential expression of bax, bcl-2 and osteopontin by fluoride in the renal tubular cells in vitro.</p><p><b>METHODS</b>The renal tubular cells were cultured and exposed to sodium fluoride (NaF) in 1, 5, 7.5, 12.5 mg F-/L level. The transcription level of bax, bcl-2 and osteopontin were investigated by reverse transcription - polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The expression of bax mRNA in 7.5 and 12.5 mgF-/L groups (optical absorption ratio value was 2.37 +/- 0.18 and 2.64 +/- 0.19 respectively) was significantly increased (P < 0.01). On the contrary, the level of bcl-2 obviously decreased (5 mg F-/L group optical absorption ratio value, 0.80 +/- 0.22, P < 0.05; 7.5 mg F-/L group optical absorption ratio value 0.71 +/- 0.22, P < 0.01). The expression mRNA of osteopontin was significantly increased when cells were exposed to fluoride at 7.5 mg F-/L (optical absorption ratio value 2.01 +/- 0.40 P < 0.01), in that group the tubular cell apoptotic trend was obvious.</p><p><b>CONCLUSION</b>NaF might induce tubular cell apoptosis via activation of bax expression and bcl-2 suppression. Osteopontin might protect the tubule against apoptosis in a lower fluoride level, but the function should be decreased in higher fluoride level.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sodium Fluoride
/
RNA, Messenger
/
Cells, Cultured
/
Rats, Wistar
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Expression Profiling
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2005
Type:
Article
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