Effect of conjugated linoleic acid on gene expression of adiponectin of obese rat fed with high fat diet / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 33-36, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-299200
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of conjugated linoleic acid (CLA) on expression of adiponectin in white adipose tissue of obese rats.</p><p><b>METHODS</b>Male Wistar rats were randomly divided into control group, high-fat group and high fat + CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight), we observed the effect of CLA on serum insulin and glucose levels of obese rats, and the reverse transcription polymerase chain reaction (RT-PCR) technique was used to measure the expression level of adiponectin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA.</p><p><b>RESULTS</b>The serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) microIU/ml, (5.09 +/- 0.66) mmol/L. The supplement of CLA decreased the hyperinsulinemia and hyperglycemia, the serum insulin in CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight) were (6.99 +/- 1.77) microIU/ml, (7.36 +/- 1.48) microIU/ml, (7.85 +/- 1.60) microIU/ml (P < 0.05), and glucose were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L (P < 0.05), (4.06 +/- 0.63) mmol/L (P < 0.05), CLA can increase the expression of adiponectin and PPARgamma in adipose tissue of obese rat.</p><p><b>CONCLUSION</b>The CLA might improve the insulin resistance of the obese rat and increase the expression of adiponectin mRNA, which might possibly act through activating PPARgamma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Insulin Resistance
/
RNA, Messenger
/
Random Allocation
/
Adipose Tissue
/
Rats, Wistar
/
Reverse Transcriptase Polymerase Chain Reaction
/
Linoleic Acids, Conjugated
/
PPAR gamma
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS