α-GalCer administration after allogeneic bone marrow transplantation improves immune reconstitution in mice / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 91-97, 2011.
Article
in English
| WPRIM
| ID: wpr-299407
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of α- galactosyleramide( α-GalCer ) on immune reconstitution under acute graft-versus-host disease (aGVHD).</p><p><b>METHODS</b>BALB/c mice were transplanted wit hallogeneic C57BL/6 bone marrow cells and splenocytes (both 1×10(7))after receiving lethal total-body irradiation. α-GalCer (100 ug/kg) or vehicle (dimethylsulfoxide) was administered intraperitoneally immediately after transplantation. The effects of α-GalCer on immune reconstitution,proliferation of T cells and B cells, hematopoiesis,and thymic microenvironment were assessed.</p><p><b>RESULTS</b>The α-GalCer group exhibited higher percentages of CD3(+),CD4(+), CD8(+), B220(+), CD40(+), and CD86(+)cells compared with the vehicle group . The number of colony forming unit per 1000 CD34(+) cells in the α-GalCer group was higher than in the vehicle group ( P=0.0012).In vitro proliferation assays showed that the α-GalCer group had higher percentages of CD3(+), CD4(+), CD8(+),and B220(+) cells compared with the vehicle group. As for the results of in vivo proliferation assays, the numbers of CD3(+), CD4(+), CD8(+), and B220(+)cells were higher in the α-GalCer group than in the normal group ,especially the number of B220(+) cells ( P=0.007).Significant difference was not found in thymocyte count between the α-GalCer group and the vehicle group, nor in the percentages of CD3(+), CD4(+), and CD8(+) cells.</p><p><b>CONCLUSION</b>Administration of α-GalCer after allogeneic bone marrow transplantation may promote immune reconstitution in the presence of aGVHD.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Transplantation, Homologous
/
Hematopoietic Stem Cells
/
B-Lymphocytes
/
Lymphocyte Activation
/
T-Lymphocytes
/
Bone Marrow Transplantation
/
Allergy and Immunology
/
Galactosylceramides
/
Graft vs Host Disease
Limits:
Animals
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2011
Type:
Article
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