Your browser doesn't support javascript.
loading
Myocardial toll like receptor 4 expression in a rat model of myocardial ischemia reperfusion injury / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 57-61, 2008.
Article in Chinese | WPRIM | ID: wpr-299498
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of TLR4 in myocardial ischemia reperfusion injury (MI/RI) by observing the dynamic TLR4 expression changes at mRNA and protein levels early after myocardial ischemia reperfusion.</p><p><b>METHODS</b>Male SD rats were randomly divided into Sham and IR group and the rats were killed according to different reperfusion time (0, 0.5, 1, 2, 4 and 8 hours). Myocardial changes under light microscope and transmission electronic microscope were observed. TLR4 expressions at protein and mRNA levels were detected by immunohistochemistry and realtime RT-PCR respectively. Myocardial TNF-alpha was determined by ELISA.</p><p><b>RESULTS</b>(1) Myocardial injury was observed in IR but not in Sham group and histopathological and ultrastructural changes in IR group remained unchanged up to 8 hours after reperfusion. (2) Positive TLR4 protein staining was visualized in both Sham and IR groups and significantly increased and peaked at 1 hour of reperfusion in IR group. (3) Compared to Sham group, TLR4 mRNA level was upregulated in myocardium in IR group and peaked at 1 hour of reperfusion. (4) Concentration of TNF-alpha in IR group was significantly higher than that of Sham group at corresponding time points (all P < 0.05), and myocardial TLR4 mRNA level correlated positively with myocardial TNF-alpha (r = 0.728, P < 0.01).</p><p><b>CONCLUSION</b>Expression of TLR4 in myocardium during early after myocardial ischemia reperfusion was upregulated and actived TLR4 might play an important role in MI/RI through promoting myocardial TNF-alpha excretion.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Myocardial Reperfusion Injury / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley / Disease Models, Animal / Toll-Like Receptor 4 / Metabolism / Myocardium Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Cardiology Year: 2008 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Myocardial Reperfusion Injury / Tumor Necrosis Factor-alpha / Rats, Sprague-Dawley / Disease Models, Animal / Toll-Like Receptor 4 / Metabolism / Myocardium Type of study: Prognostic study Limits: Animals Language: Chinese Journal: Chinese Journal of Cardiology Year: 2008 Type: Article