Down-regulated expression of UNC5b related to hepatocellular carcinoma angiogenesis / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between UNC5b gene expression and angiogenesis of hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>In situ hybridization was performed to detect the expression of UNC5b mRNA in HCC samples, paracarcinomatous liver tissues samples and normal liver samples. The relationship between UNC5b mRNA expression and the HCC clinicopathological features were also analyzed. Human umbilical artery endothelial cells were isolated and stimulated with HCC tissues homogenate, vascular endothelial growth factor and basic fibroblast growth factor. Then RT-PCR was employed to detect the expression of UNC5b mRNA in normal HUAEC as well as activated HUAEC.</p><p><b>RESULTS</b>In situ hybridization results showed that UNC5b mRNA expression was detected majorly in endothelial cells of all normal liver tissues, and partial PCLTs but was weak or even undetectable in endothelial cells of the corresponding HCC tissues. The expression levels of UNC5b gene in PCLTs were significantly correlated with capsular formation of HCC. Furthermore, RT-PCR results showed that the expression levels of UNC5b mRNA in activated HUAEC were significantly higher than those in normal HUAEC.</p><p><b>CONCLUSIONS</b>Down-regulation of UNC5b gene expression is related to angiogenesis of HCC, which may be associated with the progression of HCC.</p>