Synthesis and identification of artificial antigens of paneoniflorin / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2043-2046, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-299834
ABSTRACT
Oxidation method with sodium iodide was used to synthesize immunogenic antigen (PF-BSA) and coating antigen (PF-OVA) of paeoniflorin. UV spectroscopy showed that paeoniflorin was successfully conjugated with BSA and OVA. After immunized by PF-BSA, the mice can produce anti-paeoniflorin antibodies specifically. The ELISA test results showed the high titer (112 800) and specificity (IC50 = 0.791 mg x L(-1)) of the antiserum from mice injected with PF-BSA. Also, the antiserum showed low cross activities against nine traditional Chinese medicine (TCM) of small molecules. These artificial antigens were successfully synthesized and the anti-paeoniflorin antibody well prepared, which provides the experimental basis for the further study of ELISA and its kit.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Serum Albumin, Bovine
/
Enzyme-Linked Immunosorbent Assay
/
Drugs, Chinese Herbal
/
Chemistry
/
Monoterpenes
/
Allergy and Immunology
/
Glucosides
/
Mice, Inbred BALB C
/
Antibodies
/
Antigens
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article
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