The study on correlation of c-myc gene expression with vascular smooth muscle cell proliferation in patients with portal hypertension / 中华外科杂志
Chinese Journal of Surgery
;
(12): 543-545, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-299906
ABSTRACT
<p><b>OBJECTIVE</b>To investigate c-myc proto-oncogene expression and the relationship of PCNA protein expression of extrahepatic vascular smooth muscle cell in patients with portal hypertension and normal vessels.</p><p><b>METHODS</b>RT-PCR was used to demonstrate the expression of c-myc mRNA and immuno-chemistry strain was performed to detect the expression of PCNA protein in splenic veins of 28 patients with portal hypertension and 12 normal vessels.</p><p><b>RESULTS</b>The straining of PCNA protein was (29.8 +/- 4.7)% in splenic veins with portal hypertension, Normal vessels did not detect PCNA protein expression (P < 0.01); RT-PCR showed that the expression of c-myc mRNA in PCNA-positive control and in negative control of splenic veins with portal hypertension were (7.61 +/- 1.04)% and (3.82 +/- 0.92)%, respectively. There ws different between two groups (P < 0.01) and significant different (P < 0.01) when compared with (1.01 +/- 0.21)% in normal vessels.</p><p><b>CONCLUSIONS</b>The c-myc was immediate-early gene when it modulated proliferation of vascular smooth muscle cell. Hemodynamic disturbance of portal vein system activate the proto-oncogene of smooth muscle cells in splenic vein of patients with portal hypertension, promoting the proliferation, migrating and phenotypic change and resulting in vascular remodelling of splenic veins.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Splenic Vein
/
In Vitro Techniques
/
RNA, Messenger
/
Gene Expression
/
Genes, myc
/
Proliferating Cell Nuclear Antigen
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Cell Proliferation
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2004
Type:
Article
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