Effects of substance P on granulation tissue fibroblasts proliferation and expression of basic fibroblast growth factor mRNA / 中华外科杂志
Chinese Journal of Surgery
;
(12): 366-368, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-299915
ABSTRACT
<p><b>OBJECTIVE</b>To explore the proliferation-promoting effect of sensory neuropeptide substance P (SP) on the cultured granulation tissue fibroblasts in vitro and its regulative effect on the gene expression of basic fibroblast growth factor (bFGF) mRNA.</p><p><b>METHODS</b>The proliferation-promoting effect of cultured granulation tissue fibroblasts was observed by means of MTT; the regulative effect of SP on gene expression of fibroblast bFGF by RT-PCR. The time and dose-efficiency relations were also observed.</p><p><b>RESULTS</b>There was a significant proliferation-promoting effect of SP on the cultured granulation tissue fibroblasts in vitro in a remarkable dose-dependent fashion. However, bFGF antibody only partly exerted its inhibitive effect. SP could induce the bFGF mRNA expression of the fibroblasts at the 3rd and 6th hour (P < 0.01). SP could promote the bFGF mRNA expression of the fibroblasts in the concentration of 10(-9) - 10(-5) mol/L and peaked in the concentration of 10(-7) mol/L.</p><p><b>CONCLUSIONS</b>SP has a significant proliferation-promoting effect on the granulation tissue fibroblasts, which is correlated with SP inducing bFGF mRNA expression of fibroblasts.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Substance P
/
Gene Expression
/
Cell Division
/
Fibroblast Growth Factor 2
/
Rats, Wistar
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Dose-Response Relationship, Drug
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2004
Type:
Article
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