Construction of eucaryotic expression plasmid carrying the BMP7 gene and expression in mesenchymal stem cells / 中华外科杂志
Chinese Journal of Surgery
;
(12): 445-448, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-300011
ABSTRACT
<p><b>OBJECTIVES</b>To construct an eucaryotic expression plasmid carrying the BMP7 gene and express in MSCs.</p><p><b>METHODS</b>The BMP7 gene was cloned into the eucaryotic expression vector pcDNA3.1. At the same time, mesenchymal stem cells (MSCs) were isolated and cultured in vitro. The plasmid carrying the BMP7 gene was transfected into MSCs.</p><p><b>RESULTS</b>PCR and digesting demonstrated that the eucaryotic expression plasmid -pcDNA-BMP7 was obtained. RT-PCR and immunohistochemical methods showed that the BMP7 gene was expressed in MSCs.</p><p><b>CONCLUSION</b>Construction of an eucaryotic expression plasmid carrying BMP7 gene and expression in MSCs provide a sound basis for gene therapy using the BMP7 gene and the ideal seeds for tissue engineering.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Genetic Therapy
/
Polymerase Chain Reaction
/
Transforming Growth Factor beta
/
Bone Morphogenetic Proteins
/
Tissue Engineering
/
Bone Morphogenetic Protein 7
/
Mesenchymal Stem Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2003
Type:
Article
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