Clinical significance of CEA mRNA detection in pleural and peritoneal effusions by real-time fluorescent quantitative RT-PCR / 中华外科杂志
Chinese Journal of Surgery
;
(12): 411-413, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-300021
ABSTRACT
<p><b>OBJECTIVES</b>To detect CEA mRNA levels in benign and malignant pleural and peritoneal effusions and evaluate their clinical significance.</p><p><b>METHODS</b>Samples of pleural and peritoneal effusions from 58 patients with malignant diseases and 76 patients with benign diseases were collected and total RNAs were prepared and subjected to real-time fluorescent quantitative RT-PCR to determine the CEA mRNA levels in these samples. The positive rate of this examination was compared with that of shed cell pathological examination.</p><p><b>RESULTS</b>Nineteen samples (32.8%) of pleural and peritoneal effusions from the 58 patients with malignant diseases showed positive results in shed cell examination, while the number of CEA mRNA >1 CN was 46 (79.3%) (chi(2) = 21.81, P = 0.000). Nineteen samples of pleural and peritoneal effusions from the 76 patients with benign diseases showed CEA mRNA > 1 CN (25.0%), which was significantly different from that of the patients with malignant diseases (chi(2) = 38.85, P = 0.000).</p><p><b>CONCLUSION</b>CEA mRNA levels in pleural and peritoneal effusions can be quantified by real-time fluorescent quantitative PCR, which is more sensitive than shed cell pathological examination. This technique is helpful in discrimination of benign and malignant pleural and peritoneal effusions.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pleural Effusion
/
Ascitic Fluid
/
RNA, Messenger
/
Carcinoembryonic Antigen
/
Chemistry
/
Pleural Effusion, Malignant
/
Reverse Transcriptase Polymerase Chain Reaction
/
Fluorescence
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2003
Type:
Article
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