Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray / 中华外科杂志
Chinese Journal of Surgery
;
(12): 180-182, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-300055
ABSTRACT
<p><b>OBJECTIVE</b>To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.</p><p><b>METHODS</b>The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.</p><p><b>CONCLUSIONS</b>cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Aldehyde Reductase
/
Drug Resistance, Neoplasm
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Drug Therapy
/
Genetics
/
Liver Neoplasms
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2003
Type:
Article
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