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Study on hydrolysis of baicalin into baicalein by immobilized beta-glucosidase in a two-phase system / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 669-673, 2014.
Article in Chinese | WPRIM | ID: wpr-300171
ABSTRACT
The optimum conditions of baicalin hydrolysis into baicalein by immobilized beta-glucosidase in a two-phase system was studied and the yield was observed. A two-phase system comprising of sodium acetate buffer and chloroform was determined by comparing the solubleness of baicalein in different solvents and partition coefficient of baicalein in related aqueous-organic two-phase system. beta-Glucosidase was immobilized by the crosslinking-embedding method using sodium alginate as the carrier The optimum reaction temperature, pH value, Michaelis constant, the thermal stability and pH stability were assayed. By comparing the yield of baicalin hydrolysis into baicalein by immobilized beta-glucosidase in two-phase system, the optimum reaction conditions were determined-the optimum reaction temperature, pH value and time were 50 degrees C, 5.0 and 10 h, respectively. The yield of baicalein was 85.28%. Compare with one-phase system, two-phase system had an advantage in reaction rate and yield.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Flavonoids / Enzyme Stability / Drugs, Chinese Herbal / Chemistry / Beta-Glucosidase / Flavanones / Enzymes, Immobilized / Biocatalysis / Hydrolysis Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Flavonoids / Enzyme Stability / Drugs, Chinese Herbal / Chemistry / Beta-Glucosidase / Flavanones / Enzymes, Immobilized / Biocatalysis / Hydrolysis Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2014 Type: Article