Activating protein-1 members in response to changes of wall-shear stress in osteoblastic cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe activating protein-1 (AP- 1) members in response to changes of wall-shear stress in osteoblastic cells in vitro.</p><p><b>METHODS</b>Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and subcultured. The third generation cells were subjected to wall-shear stress of 0.8 Pa, 1.2 Pa, 1.4 Pa and 1.6 Pa separately. Gene expression of the seven AP-1 members were studied before (0 h) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.</p><p><b>RESULTS</b>The expression of FosB, c-Fos, c-Jun, JunD and JunB mRNA increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells compared to 0.8 Pa , 1.4 Pa and 1.6 Pa stress, and peaked at 15 min.</p><p><b>CONCLUSION</b>Mechanical environment changes in osteoblastic cells induced a dramatic induction of most of the AP-1 members.</p>