Comparison of ALS gene mRNA between sessile and planktonic Candida albicans of oral cavities / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 233-236, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-300326
ABSTRACT
<p><b>OBJECTIVE</b>To observe the different mRNA levels of Candida albicans ALS gene family between planktonic and biofilm-grown cells.</p><p><b>METHODS</b>ATCC 90038 and a wild strain of Candida albicans, biofilm models in vitro were formed on glass slides. After 48 hours' incubation, the biofilm-grown cells were harvested. Half-quantification of ALS1 and ALS4 mRNA was based on the amplification by one-step RT-PCR.</p><p><b>RESULTS</b>The amounts of ALS1 and ALS4 mRNA of the wild strain in biofilm increased comparing with planktonic cells, while ATCC 90038 didn't.</p><p><b>CONCLUSION</b>The members of ALS gene family may play important roles in the course of Candida albicans biofilm formation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Candida albicans
/
RNA, Messenger
/
Fungal Proteins
/
Biofilms
/
Microbiology
/
Mouth
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2005
Type:
Article
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