Effect of alpha-synuclein on the promoter activity of tyrosine hydroxylase gene / 神经科学通报·英文版
Neuroscience Bulletin
;
(6): 53-57, 2007.
Article
in English
| WPRIM
| ID: wpr-300998
ABSTRACT
<p><b>OBJECTIVE</b>To approach the associated mechanism by which alpha-synuclein (alpha-Syn) might regulate the metabolism of dopamine.</p><p><b>METHODS</b>A DNA fragment, located at -495 to +25 of the human tyrosine hydroxylase (TH) gene, was amplified by PCR and inserted into the pGL(3)-Basic luciferase reporter vector. The recombinant plasmid pGL(3)-THprom was transfected into a dopaminergic cell line MES23.5 or a alpha-Syn over-expressed MES23.5 (named MES23.5/halpha-Syn(+)). The promoter activity was detected by the Dual Luciferase Assay System.</p><p><b>RESULTS</b>The luciferase activities in the MES23.5 cells transfected with pGL(3)-Basic, pGL(3)-THprom, and pGL(3)-Control vectors were 5.60+/-0.67, 26.80+/-4.11, and 32.90+/-4.75, respectively. On the other hand, the luciferase activity of pGL(3)-THprom in the MES23.5 (26.80+/-4.11) was significantly higher than that in the MES23.5/halpha-Syn(+) (14.40+/-0.61) (P<0.01).</p><p><b>CONCLUSION</b>These results indicate that the - 495 to +25 region in the TH gene possesses promoter activity for controlling the gene expression, and that alpha-Syn may negatively regulate the metabolism of dopamine by affecting the function of TH promoter as a trans-acting factor.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parkinson Disease
/
Tyrosine 3-Monooxygenase
/
Substantia Nigra
/
Transfection
/
Dopamine
/
Down-Regulation
/
Gene Expression Regulation, Enzymologic
/
Promoter Regions, Genetic
/
Genes, Reporter
/
Cell Line, Tumor
Limits:
Animals
Language:
English
Journal:
Neuroscience Bulletin
Year:
2007
Type:
Article
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