Simultaneous determination of pseudoephedrine and chlorpheniramine in human plasma by HPLC-UV detection method / 药学学报
Acta Pharmaceutica Sinica
;
(12): 281-284, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-301095
ABSTRACT
<p><b>AIM</b>To establish a sensitive and specific method to simultaneous determination of pseudoephedrine and chlorpheniramine in human plasma.</p><p><b>METHODS</b>Pseudoephedrine and chlorpheniramine were extracted from alkaline plasma with t-butyl methyl ether as the base form, and were back-extracted into 1.5% hydrochloride solution. The two drugs were simultaneous determined by RP-HPLC with ultraviolet detection at 200 nm, using dextromethorphan as internal standard. A C18 column (250 mm x 46 mm ID) and a mobile phase containing acetonitrile-water-triethylamine (46540.2, containing 10 mmol x L(-1) sodium dodecyl sulfate (SDS) and 60 mmol x L(-1) NaH2 PO4, adjusted pH to 2.6 with H3PO4) were used.</p><p><b>RESULTS</b>The limit of quantification was 10.0 and 0.5 microg x L(-1), the linear range was 1.5 - 0.01 mg x L(-1) and 75 - 0.5 microg x L(-1), for pseudoephedrine and chlorpheniramine, respectively. The within-day and between-day RSD were less than 12.4%, and the average recovery was between 97.3% - 109.4%.</p><p><b>CONCLUSION</b>The method was sensitive, specific, simple, and suitable for drug level monitoring in clinical pharmacokinetic study.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spectrophotometry, Ultraviolet
/
Blood
/
Pharmacokinetics
/
Chlorpheniramine
/
Chromatography, High Pressure Liquid
/
Delayed-Action Preparations
/
Ephedrine
/
Methods
Type of study:
Diagnostic study
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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