Absorption of recombinant hirudin in rats GI tract / 药学学报
Acta Pharmaceutica Sinica
;
(12): 77-80, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-301144
ABSTRACT
<p><b>AIM</b>To investigate the absorption and distribution of recombinant hirudin-2 (rHV2) in the GI tract in rats after oral administration.</p><p><b>METHODS</b>Using HPLC, fluorescence spectrophotometry and confocal laser scanning microscopy to measure the amount of intact rHV2 absorbed into gastrointestinal mucosa and blood.</p><p><b>RESULTS</b>HPLC spectrum showed that there were intact rHV2 molecules in plasma after 1 h of oral administration. After oral administration for 3 h, 1.2%-26.8% of fluorescence was found in the GI tract in rats. Chromatographic analysis showed that 2.27%-38.75% of fluorescence recovered from the GI tract luminal contents and mucosa was eluted at the void volume of a Sephadex G-25 column. Microscopic examination showed that FITC-rHV2 was taken up throughout the whole small intestine but the ileum appeared to be a preferred site for FITC-rHV2 transport in rats.</p><p><b>CONCLUSION</b>rHV2 may partially survive in the GI lumen and subsequently absorbed in active form by gastrointestinal tract.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Pharmacokinetics
/
Tissue Distribution
/
Random Allocation
/
Administration, Oral
/
Hirudins
/
Fluorescein-5-isothiocyanate
/
Rats, Sprague-Dawley
/
Digestive System
/
Fibrinolytic Agents
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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